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58178

Method Development and Validation of Carbendazim Fungicide in Cucumber تطوير واعتماد طريقة لتحليل المبيد الفطرى الکاربندازيم فى الخيار

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Last updated: 04 Jan 2025

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Abstract

Evaluation of pesticide residues was carried out in cucumber to determine the residues of carbendazim which collected from different local markets in Egypt. During a 14-day, treated cucumbers samples were gathered and analyzed to determine the carbendazim residue quantity.  A QuEChERS sample preparation has been applied with liquid chromatography provided with mass spectrometry (LC/MS-MS) to evaluate residual of tested fungicide. Two transitions ions was obtained from multiple reaction monitoring (MRM) after that higher sensitivity transition used for quatification but the lower sensitivity transitions used to confirmation analysis of carbendazin. The method was validated by setting performance parameters such as linearity, precision, recovery, limits of detection (LOD) (0.45 µg Kg1) and limits of quantification (LOQ) (0.9 µg Kg1). Good correlation coefficient R2 0,9992 in pure solvent curve and R2 1.000 for matrix-matched curve, which given the elevated calibration curve quality. The carbendazin recoveries founded in fresh cucumber samples of 10 replicates were found to be in the range between 119.11 to 124.45% (% RSD > 3.5) for two fortification levels (0.010 and 0.070 mgKg1). The intra-day repeatability RSD value was 3.26%, while the inter-day repeatability RSD value was 5.27%. Matrix effect of carbendazim in cucumber was evaluated and the results showed that carbendazim had a soft matrix effect (6.53).Samples of tested fungicides showed distinct contamination levels up to a certain time period (7 days), after which no residues were identified between 11-14 days.

DOI

10.21608/jppp.2019.58178

Keywords

Pesticide residue, Ultra Performance Liquid Chromatography, MS Detector, Carbendazim

Authors

First Name

علاء

Last Name

خزيمى

MiddleName

مسعود

Affiliation

قسم وقاية النبات - کلية الزراعة - جامعة دمنھور - مصر

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Orcid

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First Name

محمد

Last Name

رمضان

MiddleName

فتحی عبد الرحمن

Affiliation

مرکزالبحوث الزراعية - المعمل المرکزي للمبيدات - قسم بحوث تحليل المبيدات - الدقي - الجيزة – مصر

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Volume

10

Article Issue

8

Related Issue

8801

Issue Date

2019-08-01

Receive Date

2019-07-05

Publish Date

2019-08-01

Page Start

397

Page End

400

Print ISSN

2090-3677

Online ISSN

2090-3758

Link

https://jppp.journals.ekb.eg/article_58178.html

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https://jppp.journals.ekb.eg/service?article_code=58178

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2

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Original Article

Type Code

888

Publication Type

Journal

Publication Title

Journal of Plant Protection and Pathology

Publication Link

https://jppp.journals.ekb.eg/

MainTitle

Method Development and Validation of Carbendazim Fungicide in Cucumber تطوير واعتماد طريقة لتحليل المبيد الفطرى الکاربندازيم فى الخيار

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Article

Created At

22 Jan 2023