Beetroot (Beta vulgaris L.) have been used for many years as a food supplement to support treating a number of diseases. It is also classified as medicinal plant because of its excellent source of phytochemicals including antioxidants, minerals, vitamins and amino acids. Beetroot are vegetable belong to the family Chenopodiaceae. In food industry they are formulated in edible powder and energetic juice. In some regions of the world beetroot leaves are cut off and discard in spite of their health benefit because of lack of knowledge among the local people regarding their health benefit. So far, this investigation aimed to evaluate the possible role of the formulated antioxidant GAWDA^® on enhancing the phytochemicals of the content of beetroot leaves. P.P, Proline, Potassium and the antioxidant activity were determined. Beetroot seeds were soaked in water solution of GAWDA^® formulation (2g/L for 12 hours) pre sowing vs tap water in case of the check. The target physiological characters were recorded in leaves of 3 month old plants. The results showed that soaking seeds in water solution of GAWDA^® formulation (2g/L for 12 h) before sowing significantly increased the contents of Ch. A, B, carotenoids, total phenolic compounds, proline, potassium and antioxidant activity in the plant leaves. The potential of the formulated antioxidant (GAWDA^®) for scaling up the contents of the phytochemicals studied beetroot leaves was concluded.