The objective of this study was to evaluate the effect of using sulphur, nitrogen and phosphorus fertilization on plant growth and chemical composition of Rosmarinus officinalis L. grown under saline irrigation water. Obtained results showed that rosemary have been affected significantly with all fertilizer treatments. This reflected on increasing plant growth, nutrient contents as well as increasing total carbohydrates, total soluble sugars, reducing sugars and sucrose. The essential oil percent was particularly high with different fertilizer treatment as compared with control (saline water at 4000 ppm. NaCl only). The application of sulphur, nitrogen and phosphorus fertilization showed a marked quantitative difference in chemical composition of oil and the principal component 1,8-cineole reached the greatest amount (60.15%) with applying the medium rate (N2) of nitrogen; followed by champhor (30.56%) resulted from adding the low level (P1)of phosphorus, then b-pinene (15.32, 15.31 and 12.29%) and limonene (16.78, 17.87 and 15.91%) recorded the greatest increase with application of sulpher at medium rate (S2) and high rate of nitrogen and phosphorus (N3 and P3) fertilizers respectively.