Catharanthus roseus plants were treated with four different rates of nitrogen fertilizer (0, 28, 56, and 84 kg N/fed) and eight biofertilizer treatments (untreated, Azotobacter, Microben, Nitroben, phosphate solubilizing bacteria (PSB), Azotobacter + PSB, Microben + PSB, and Nitroben + PSB) either individually or in combined treatments. The obtained data indicated that nitrogen chemical fertilizer, when used individually, significantly increased the vegetative growth and enhanced the chemical composition of Catharanthus roseus. The medium rate of chemical fertilizer (56 kg N/fed) gave the best results.                 The use of biofertilizers alone led to a significant increase in vegetative growth and chemical composition of the tested plants as compared with the control treatment. The best biofertilizer treatment was Azotobacter + PSB. The interaction between chemical fertilizer and biofertilizer was also significant. It should be noted that the use of biofertilizers led to a significant increase in the efficiency of chemical fertilizers. The addition of low rates of chemical fertilizer (28 kg N/fed) in combination with biofertilizer gave the best results. From these results it is advisable from the economic and environmental point of view to use low rate of chemical fertilizer (28 kg N/fed) with selected biofertilizers.