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246126

DNA POLYMORPHISM FOR EVALUATION OF FOUR PEPPER G9J- TIVARS FO~ RESISTANCE TO Meloidogyne incognita AND Rotylenchulus reniformis NEMA TOOES

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Last updated: 04 Jan 2025

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Abstract

The reaction of four pepper cultivars (California Wonder, Long Green, Hot
Green and strain-9852-17), and their plant susceptibility to inoculum relation with
either Meloldogyne incognita or Rotylenchulus reniformis was studied under
greenhouse conditions during 2002 and 2003 seasons. Dala showed thaI strain-9852-
17 CV. was rated as highly resistant (HR) to root-knot nematode, M. incognita and
reniform nematode R. renlformis. White, California Wonder and Long Green cultivars
were rated as susceptible (S) for M. incognita and R. reniformis. Whereas, Hot Green
pepper cv. was rated as slightly resistant (SR) to M. incognita and R. reniformis. It was
noticed that phenolic compounds had a high concentratlon in un·infected pepper than
inoculated by either tested nematode. The percent age of phenol reduction was higher
when the pepper infected with M. incognita than R. reniformis. On the other hand, this
eVidence was conformed at the molecular level where there are the same differences
batween the four tested cultivars (California Wonder, Long Green, Hot Green and
slrain-9852-17) by using RAPD analysis. Amplification of genomic DNA from four
pepper cultivars indicated that some bands were common to all cultivars but variations
were also existing. The differences were not only in the major bands but also in some
minor bands. In primer OP-MO showed total of five specifiC bands. One band can be
used as a ma~er on position 950-bp in variety (strain-9852-17) that has highly
resistant to M. incognita and R. reniform/so Also, in primer OP-k11 Showed total of
eight specific bands. Three bands absent can be used as a marker in cullivar (strain-
9852·17) on pOSition 2SO-bp ,3QO.bp and 400-bp that have highly resistant and one
band on poSition 1500-bp in one cultivar (Hot Green) which ranked as slightly
resistant to both nematode specles. Whereas, in primer OP·B5 showed total of five
specifIC bands can be used as a marker in one cultlvar strain-9852-17 in three position
compared with those of susceptible remaining cultivars to M. incognita and R.
reniformis (California Wonder and long Green) which probably have carrying a
resistance gene to nematodes in this position.

DOI

10.21608/jpp.2003.246126

Keywords

Genome mapping, Meloidogyne incognita, pepper cultivars, RotylenchuJus reniformis

Authors

First Name

A.

Last Name

Amin,

MiddleName

W.

Affiliation

Dept 0f Agrlc. Zool. and Nematol.

Email

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City

-

Orcid

-

First Name

A.

Last Name

Gharib

MiddleName

A.

Affiliation

Dept. of Vegetable Crops, I Fac. of Agrlc., Cairo University.

Email

-

City

-

Orcid

-

First Name

Mona

Last Name

AI-Shalaby

MiddleName

E. M.

Affiliation

Dept 0f Agrlc. Zool. and Nematol.

Email

-

City

-

Orcid

-

Volume

28

Article Issue

11

Related Issue

35242

Issue Date

2003-11-01

Receive Date

2003-10-23

Publish Date

2003-11-01

Page Start

6,663

Page End

6,678

Print ISSN

2090-3669

Online ISSN

2090-374X

Link

https://jpp.journals.ekb.eg/article_246126.html

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https://jpp.journals.ekb.eg/service?article_code=246126

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5

Type

Original Article

Type Code

887

Publication Type

Journal

Publication Title

Journal of Plant Production

Publication Link

https://jpp.journals.ekb.eg/

MainTitle

DNA POLYMORPHISM FOR EVALUATION OF FOUR PEPPER G9J- TIVARS FO~ RESISTANCE TO Meloidogyne incognita AND Rotylenchulus reniformis NEMA TOOES

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Article

Created At

22 Jan 2023