The current study focuses on the evaluation of the antimicrobial activities of some Egyptian wild mushrooms: two strains of Agaricus namely; Agaricus bitorquis (A40), Agaricus bisporus (A60), and three species of Ganoderma (G1, G3, G7). They were investigated against 13 pathogenic microorganisms (eight fungal spp and five bacterial spp). The mycelia of A40, G7, and G3 were the most effective on the largest number of pathogenic microorganisms. Antimicrobial activities of the crude extracts of (A40, G7, G3) by different solvents (methanol, water & ethylacetate) were examined by the agar well diffusion method. The results revealed that these mushrooms have potential activities as natural antifungal and antibacterial. The highest antagonistic effect of mushroom extracts was exhibited by ethylacetate extract of Ganoderma (G7) against F. oxysporum and Ganoderma (G3) is the most effective against Salmonella typhi. The mushroom extracts have lower antifungal and antibacterial activites comparing with antifungal except ethylacetate extracts of both Ganoderma (G3) and Agaricus bitorquis (A40) exhibited higher inhibition effect against Curvularia sp.