The work to be described here was carried out to separate and identify the
phenolic acids of different types of garlic cloves produced in Egypt. as well as
studying the possibility of using these compounds as antioxidants by adding them to
the sunflower oil at levels of 200. 300 and 400 ppm. The second target of this study is
to evaluate the aforementioned compounds microbiologically, The antioxidative
effectiveness of phenolic compounds was compared with butylated hydroxy toluene
(6H1). The antimicrobial activity of garlic cloves fixed oil was also assayed against
some selected microorganisms by disc·diffuslon method. HPLC analysis indicated
that methanolic extracts of garlic cloves contained large number of phenoric acids,
where more than ten phenolic acids were identified. Generally, garlic cloves
methanolic extracts contained high levels of gallic, P-hydroxbenzoic, tannic, caffeic
and rerullc acids. Moreover, the results of peroxide values (PV,s) indicated, that
melhanollc extracts from different types of garlic cloves showed stronger antiQl(idative
activity Ihan thai of BHVThe antimicrobial effects of garlic cloves fixed oil were
compared with phenol (f and 10%). The diameters of the inhibition zones (mm) were
taken as criterion of antimicrobial spectra. However, the data indicated that all
samples of garlic cloves oils possess stronger antimicrobial activity than that of 1%
phenol, In general, the data showed that, Bacillus sublills was the more sensitive
microorganisms to garlic cloves oil at the most applied concentrations, where
Pseudomonas spp < /em>. was the second sensitive one, while fungi strains were the lowest
sensitive to the aforementioned material at the most applied concentrations. From the
above mentioned results, it could be concluded that garlic cloves, which used as a
spice, flavouring agent and in Egyptian salad dishes, have available constituent~.
which can be used as vital elements and acts as a microbial balancer in the
alimentary canal. However, the antioxidant properties of garlic cloves extract and the
fixed oil clarify Ihe possibilily of using them In the medicinal purposes.