Pasteurellosis is a debilitating disease occur in man and many animals due to either endogenous or exogenous infection with Pasteurella microorganisms especially under stress condition, so this study was done to detect the prevalence of such microorganism in the nasal swabs and blood samples to detect their antibodies. 105 samples were collected from apparently healthy rabbits and 154 samples from diseased ones. - The obtained data revealed that the prevalence rate of Past. muitocida was (4.8%) from apparently healthy rabbits and (12.3%) in diseased ones. - Biochemical reactions of isolated Past. microorganisms revealed that all isolates (24) were belonged to Past. muitocida. » All isolates of Past. muitocida were tested for their pathogenicity to mice with characteristic P.M.lesions. sever haemorrhage in subcutaneous blood vessels, septicaemia and congestion of the internal organs. — Serodiagnosis by using ELISA test to detect the antibody titer of Past. muttocida and the obtained data revealed that (31.4%) cf tested apparently healthy rabbits were ELISA seropositive while it was (48.1%) In diseased ones. - Concerning the antibiotic sensitivity test, it was found that all isolates were 100% sensitive to Ceftiofur sodium, Norflxacin, Gentamicin, highly sensitive to Enrofloxacin (95.8%) Sulphamethoxazoie and Trimethoprim (87.5%) while complete resistance to chloramphenicol.