Fifty-two out of one hundred and forty-four isolates of filamentous fungi were recorded as amylase producer, but with different degrees, on solid plate method. Chemical constituents of potato waste samples (collected from the Chpis' Factory for Food Industries, Assiut, Egypt) were determined by chemical analysis. Twelve isolates (eight highly producer isolates and four isolates isolated from potato wastes) were screened for amylase production on potato wastes. Aspergillus flavus443 was recorded as the best enzyme producer on potato wastes and synthetic medium. The best environmental and nutritional conditions for  amylase production by Aspergillus flavus443 were : [50 gm of potato wastes with 10 ml distilled water , manitol (1%) as a carbon  source , casein (1%) as a nitrogen source , pH 5  and the medium was incubated at 40 ºC  for three days ).