The present study was carried out to evaluate the anticancer activities of strawberry (Fragaria x ananassa)  fruits extracts. The methanolic (80%) extract was evaporated to dryness, the residue (crude extract) (CE) was fractionated by using different solvents producing, ethyl acetate fraction (EAF) and butane fraction (BUF). Completely maturated fruits moisture, sugars, fiber,  pH value, vitamin C and minerals including K , P, Ca, Mg and Na were measured and the values established as , 88.9% , 6.2% , 2.2 % , 4.2, 60 (mg/100g), 160 (mg/100g), 23 (mg/100g), 22 (mg/100g), 12( mg/ 100g), and 3 (mg/100g) respectively. EAF extract contained 43.13%, 43.15% and 2.33% from phenols, flavonoids and anthocyanins respectively, while, BUF extract which contained 37.34% ., 44.64% and 2.21% respectively, and CE extract contained 26.19%, 21.92% and 0.13% respectively .  The results revealed that all extracts of strawberry fruits were the most effective as anticancer agent since then activity ranged from 70.1 to 81.7 % at concentration of 10 μg/ml .