A study was carried out to evaluate the anticancer activities of red roomy grape (whole fruit, seeds and leaves) extracts. The methanolic (80%) extract was evaporated to dryness, the residue (crude extract) (CE) was fractionated by using two solvents, i.e. ethyl acetate fraction (EAF) and butane fraction (BUF). EAF extract contained total phenol compounds 55.03%, 29.90% and 5.40%, while BUF extract contained 52.06%, 33.65% and 8.10% and CE contained 43.39%, 20.93% and 1.70% in whole fruit, seeds and leaves respectively. The results revealed that all extracts of red roomy grape (whole fruit, seeds and leaves) were effective as anticancer agent since their activities ranged from 70.05% to 79.91%, 54.34% to 61.22% and 18.15% to 42.50% at concentration of 10 μg/ml in whole grape sample, seeds and leaves respectively.