Biological evaluation of fresh wheat grass juice and its blends were performed using 60 male albino rats. Rats were adapted for 10 days and divided into 10 groups in each group 6 rats, feeding for 6 weeks was carried out to identify the effect of fresh wheat grass juice and its blends on high cholesterol test rats. From results it could be noticed that the lowest weight increase for rats and feed consumed was found in the first group, and these results showed significant differences between them and the other groups. The infected group rats (G2) that fed on a diet containing high percentage of fat and 1% cholesterol  had the highest  level of total cholesterol and low-density lipoprotein LDL (146  and  83.0 mg/dl), respectively. The group (G9) and (G10) which fed on high fat diets and 1% cholesterol with 5 ml dose(G9)  and 10ml dose(G10) of fresh wheatgrass juice with banana and guava / kg bw / day by mouth had a decrease of total cholesterol (79.65 and  78.00  mg/dl), LDL (33.80 and 32.0 mg/dl), total lipids (285.90 and  285.30  mg/dl) and triglyceride (86.00 and  85.70  mg/dl) in (G9) and (G10) respectively , and led to an increase in the value of high density lipoprotein (HDL) (27.13 and  27.90  mg/dl), respectively. Also the use of fresh wheat grass juice and its blends in (G9 and G10) improved liver and kidney functions as compared with the other groups.