Rhizoctonia solani causing damping –off in sugar beet, which is one of the most destructive diseases in this crop worldwide. In this work, twelve bacterial  isolates were  isolated from rhizosphere of sugar beet crop of which six isolates showed antifungal activity against these phytopathogen, were identified as Bacillus spp. , Bacillus amyloliquefaciens and Bacillus pseudomycodies by standard tests and the application of biolog system .The most efffective and selected bacterial isolate (No. 8) obtained from sugar beet rhizosphere was identified using 16S rRNA .Moreover , three species of fungi as Trichoderma spp.were successfully used by several investigators to control sugar beet damping - off . In vivo, results of seed soaking with tested B. amyloliquefaciens showed that the most effective in controlling damping –off disease(93.33%) followed byT. hamatum (90.0%). While B.pseudomycodies recorded value of survival plants (86.67%), Bacillus sp.( 6) , Bacillus sp.(1) , T.  viride , Bacillus sp.(2) , T. harzianum and Bacillus sp.(3) (76.67%, 76.67%,66.67%, 63.33%, 60.00%, 56.67% ,respectively) indicated that seed coating with T.harzianum was the most effective in controlling disease (83.33%), followed by T. hamatum, B. amyloliquefaciens, B.pseudomycodies , T. viride , Bacillus sp.(2), Bacillus sp.(1), Bacillus sp.(3)andBacillus sp.( 6) (76.67%, 70.00%, 66.67%, 60.00%, 60.00%,36.67%,  33.33%, 26.67% , respectively) in soil infested with Rhizoctonia solani.