This investigation was conducted to study the effect of feeding on hemicellulose extracted from wheat bran (HC.Wb) and carboxymethyl cellulose produced from sugar beet pulp (CMC.Sbp) at levels 30, 60 and 90% for cellulose in hypercholesterolemic (Hyper-C ) rats. Results showed that, replacing hypercholesterolemic (Hyper-C) diet with HC.Wb and CMC.Sbp significantly reduced total cholesterol (T.C), total triglycerides (T.G), low density lipoproteins (LDL-C), very low density lipoproteins (v.LDL-C), while high density lipoproteins (HDL-C) increased .Furthermore, Hyper-C diet with (HC.Wb and CMC.Sbp at level of 90% for cellulose) replacement also recorded the best and closest of HDL-C to (control–ve).Also, ALT was Significantly increased hyper-C control group (G₂). Concentration of enzyme (ALT) value in Hyper-C rats in group (G₂) was 52.73 U/L, whilst group (G₁) (control-ve) were 30.46 U/L. Feeding on Hyper-C diet  replaced with HC.Wb at levels 30,60 and 90% (G₃, G₄ and G₅) led to a more reduction in concentration value of (ALT) being 39.26, 36.88 and 35.73 U/L, respectively while feeding on a high cholesterol diet  replaced with CMC.Sbp at levels 30,60 and 90% (G₆, G₇ and G₈) led to a more reduction in concentration value of (ALT) were 37.50,35.38and 34.30 U/L, respectively comparing with Hyper-C control group (G₂). Histopathological examination showed that feeding diets supplemented with HC.Wb and CMC.Sbp to hyper-C  rats lowered the degree of liver lesions. So it can be suggested that, HC.Wb and CMC.Sbp have a clear effect in decreasing cholesterol concentration and may be beneficial for patients with liver and cholesterol diseases.