Three samples of partial purified Manna from three locations at Penjween in Sulaymaniyah governorate/ Iraq were prepared. Locations were Ahmadklwan (A), Bnawaswta (B), and Kanimang (K). HPLC has been used to determined type and rates of sugars within the Manna. Results showed that all samples A, B, and K contained a huge amount of fructose (22.3%, 21.9%, 22.6%) respectively. samples showed less glucose than fructose in their content which was (A 13.3, B 13.4, K 11.2%). Manna contained a considerable amount of sucrose and trehalose, for sucrose was 26.8%, in A, 26.9% in B, 26.0% in K. Likewise the rate of trehalose within sample A, B, and K was (36.8, 20.4, 24.2%) respectively. The existence of alcoholic sugars was very low, for instance, the quantity of sorbitol was (0.5%, 0.9%, 1.2%) for samples A, B, and K respectively, and for mannitol was 0.1% in all the samples. ICPE used to determine the rate of minerals, Calcium rate showed a significant difference among the Manna samples, which was A (25.200), B (11.000), K (16.450). As well as for iron (Fe) rate a mass variation was noticed through the samples (A 4600, B 3.785, and K 3.965). In general, magnesium (Mg) content was relatively high with the rate (4.410) in A, (3.005) in B, (3.365) in K. results confirmed that Manna no content chromium (Cr) and cadmium (Cd). Although, there was no lead (Pb) in B and K, but a very low amount appeared in A (8.55). Results are calculated in mg/kg.