The aim of the present study is to prepare Microwave defatted black rice bran (MDBRB)to study the effect of its substitution for casein on body weight, feed efficiency ratio, serum liver function enzymes, serum lipids profile and antioxidant enzymes in carbon tetrachloride (CCl₄) intoxicated rats. Results showed that substitution of MDBRB for casein especially at 75 and 100% in CCl₄ -intoxicated rats increased their feed intake and body weight gain. This substitution also decreased the levels of serum liver function enzymes, improved lipid profile and increased the activity levels of antioxidant enzymes in CCl₄ intoxicated rats. Histopathological examination revealed alleviation of hepatic lesions caused by CCl₄ by increasing the percentage of DBRB used. In conclusion, it was suggested that MDBRB could protect the liver cells from CCl₄ induced liver damages perhaps, by its antioxidative effect on hepatocytes, hence eliminating the deleterious effects of toxic metabolites from CCl₄. So the present study recommended that the use of MDBRB may be useful for patients suffering from liver diseases due to its hepatoprotective and hypolipidemic activities.