The current work aimed to study the biological effects of fresh un-pollinated date, date palm pollen and doum fruit against some cancer cells line and some pathogenic microorganisms. Chemical composition including moisture content, total soluble solids, ash content and total and reducing sugars were determined. Also, antioxidant compounds such as phenolic compounds, hydrolysable tannins, condensed tannins and flavonoids were measured. The biological effects of fresh un-pollinated date, date palm pollen and doum fruit  extracts against some human cancer cells include colon (HCT), prostate (PC3), breast (MCF7) and larynx (HEP2) cancers were tested. In addition, the antimicrobial effect of these extracts against some pathogenic microorganisms includes bacteria, mold and yeast were examined. The results indicated that fresh un-pollinated Siwi date had the highest content of antioxidant compounds compared with date palm pollen and doum fruit, therefore lead to the high antiradical activity. On the other hand, the date palm pollen had the highest content of some minerals content such as calcium, magnesium, iron, zinc and manganese compared with fresh un-pollinated date and doum fruits. The best effect against the four human cancer cells has been observed with fresh un-pollinated date extract. The cancer cells viability has been decreased up to 50% when the fresh un-pollinated date extract was used with concentration 62 µg/ml approximately. However, the highest decreased of cancer cells reached to 25% approximately when a concentration of the extracts has been used between 125 and 250 µg/ml. Also, fresh un-pollinated date revealed the best effect against gram negative bacteria but date palm pollen exhibited the best effect against gram positive bacteria, mold and yeast. So, the obtained data of the present study pointed that we neglected a very important bio-active products.