This study was performed to investigate the effect of feeding on  Microwave defatted black rice bran (MDBLRB) in hypercholesterolemic rats . Chemical composition and total phenolic compounds (TPC) of microwave defatted black rice bran (MDBLRB) and microwave full fat black rice bran (MFBLRB) were determined . Results revealed that (MDBLRB) protein, ash, fiber and carbohydrates contents of (18.20, 0.70, 10.1, 13.7 and 71.00) were higher than those of MFBLRB. Also, total phenolic compounds (TPC) being 561 as mg Tannic acid equivalent/kg for (MDBLRB) in compare with 486 as mg Tannic acid equivalent/kg for MFBLRB, obtained results illustrated that substitution of feeding hypercholesterolemic diet with MDBLRB led to improvement the High lipoprotein cholesterol HDL-C. Furthermore, hypercholesterolemic diet with (MDBLRB) replacement at 75% and 100% also recorded the best and nearest of HDL-C to the negative control. It was observed also that LDL- cholesterol value of negative control diets (G1and G2) were 46.78 and 45.15 mg/dl, but also the value of the hypercholesterolemic  in control (G3) was 198.77mg/dl. On the other hand, the LDL cholesterol of rats fed on hypercholesterolemic diet substitution with MDBLRB 25,50.75 and 100% (G4, G5, G6 and G7) being 50.67, 47.85, 46.28 and 41.73 mg/dl, respectively. At the final of experiment (10 weeks), ALT was significantly increased for the hypercholesterolemic control (G3) was 50.30 U/L, while negative control (G1 and G2) were 25.80 and 24.20 U/L respectively. Feeding on hypercholesterolemic diets substituted with MDBLRB (G4, G5, G6 and G7) led to a more reduction at level 25, 50, 75 and 100% were 38.52, 36.61, 34.34 and 32.55 U/L, respectively comparing with hypercholesterolemic control (G3). Briefly  it be could conclude  that MDBLRB has  pronounced effect in lowering cholesterol serum levels and may be useful for patients suffering from liver and cholesterol diseases