Consumption of aflatoxin contaminated food are dangerous source to human health because of their toxic, carcinogenic, hepatotoxic and mutagenic characteristics. Due to their harmful effects concerned on bring up todate the detoxification methods applied for reduction of aflatoxins by physical (adsorption), chemical (chemical compound) methods. The present study aimed to investigate the influence of some natural additives in biscuit such as dried blueberries, wheat bran and nano-charcoal as detoxifying agents which are available and inexpensive, that would modulate aflatoxin metabolism to reduce aflatoxins contamination, which enhances food safety and human health.Thirty adult male albino rats were partitioned into five groups. The first main group (n=6 rats), negative control, was fed on basal diet. The second group was fed on peanut diet contaminated with 20 ppb aflatoxin / gram (ng/g diet),   positive control, ( AFL+diet). Groups from group three to five were fed toxic diet + detoxifying agent (biscuit addition of dried blueberries or wheat bran and nano-charcoal, respectively) for 8 weeks. The results revealed the adverse impacts of aflatoxin contaminated diets on hematological alterations, lipid profile, liver functions, serum protein parameters, kidney function and hepatic and kidney histological.The lipid profile (TC, TG, HDL and LDL) were improved in treated groups compared with positive group G2. Addition of  dried blueberries or  wheat bran and nano-charcoal to biscuit  significantly (P<0.05) enhanced liver functions , also serum protein parameters (albumin, globulin and total protein) and kidney function ( creatine and urea)  in which obtained results were nearly the same of negative group  (G-ve) . But, groups exposure to aflatoxin increased the level of cholesterol, triglycerides, LDL, HDL, creatine and urea.Hence , it could be finished that efficient use of  dried blueberries or,wheat bran and nano-charcoal  to biscuit  as food additives  against the toxicity of aflatoxin may lead to decrease  their the harmful effects.