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36014

Acid Tolerance and Growth of Four Strains of Bifidobacteria in Different Types of Milk

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Last updated: 04 Jan 2025

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Abstract

Four strains of  bifidobacteria ( Bifidobacterium angulatum  ATCC 2238, Bifidobacterium animalis subsp lactis Bb12 , Bifidobacterium bifidum ATCC 2203, and Bifidobacterium bifidum  LMG 10645) were studied  for acid tolerance and growth in different types of mammalian milks . All studied strains grew at a wide range of pH , ranging from pH 2.0 - pH  6.5 .  However the growth was quite low at pH 2.0 , it was in the range  between (0.07 - 0.12  optical density at 660 nm  ) and slightly higher when the pH of the medium was raised to pH 3.0 , at this pH  the growth was between ( 0.08 - 0.18) optical density at 660 nm    . On the other hand , at pH 4.0   the optical density was ranging between ( 0.90 - 2.10  at 660 nm)   . The higher growth rate was achieved at pH 6.5 , it was between (3.55- 4.35) optical density at 660 nm. The studied strains were grown in human, cow,  goat, buffalo, sheep and camel milks. All strains were able to grow at  the six types of milk , the growth was in the range between 1.5 x106 cfu /ml (sheep milk ) -  2.1x107 cfu /ml (cow milk ) a slight decrease was noticed when Bifidobacterium angulatum 2238  was grown in sheep milk . Bifidobacterium bifidum 2203 reached the count of 10 x109 cfu /ml in goat milk and the lowest growth was noticed in human milk .  The slowest growth of Bifidobacterium animalis subsp lactis Bb12 was 7.5 x105 cfu /ml , it was found when it was grown in camel milk  and  the best growth was noticed in  goat and sheep milks . Bifidobacterium bifidum  LMG 10645 grew well in all types of milk except human milk , the growth rate was almost around 106 - 10cfu /ml,whereas a slight decrease was noticed when they grew in human milk 5.9 x106 cfu /ml.

DOI

10.21608/jfds.2018.36014

Keywords

Bifidobacteria, acid tolerance, cow, Goat, buffalo and camel milks

Authors

First Name

Raghda

Last Name

Moawad

MiddleName

M. S.

Affiliation

Dairy Department , Faculty of Agriculture , Minia university.

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Orcid

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First Name

Sabah

Last Name

Abdel-Razek

MiddleName

T.

Affiliation

Dairy Department , Faculty of Agriculture , Minia university.

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City

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Orcid

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First Name

A.

Last Name

Zahran

MiddleName

S.

Affiliation

Dairy Department , Faculty of Agriculture , Minia university.

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City

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Orcid

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First Name

F.

Last Name

Ibrahim

MiddleName

S.

Affiliation

Dairy Department , Faculty of Agriculture , Minia university

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Orcid

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Volume

9

Article Issue

7

Related Issue

5898

Issue Date

2018-07-01

Receive Date

2018-06-23

Publish Date

2018-07-10

Page Start

251

Page End

255

Print ISSN

2090-3650

Online ISSN

2090-3731

Link

https://jfds.journals.ekb.eg/article_36014.html

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https://jfds.journals.ekb.eg/service?article_code=36014

Order

5

Type

Original Article

Type Code

886

Publication Type

Journal

Publication Title

Journal of Food and Dairy Sciences

Publication Link

https://jfds.journals.ekb.eg/

MainTitle

Acid Tolerance and Growth of Four Strains of Bifidobacteria in Different Types of Milk

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Type

Article

Created At

22 Jan 2023