35866

Isolation and Identification of Antimicrobial Producing Lactic Acid Bacteria from Local Egyptian Dairy Products

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Last updated: 04 Jan 2025

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Abstract

The aime of the present work is to evaluate the antimicrobial characteristics of lactic acid bacteria (LAB) being utilized as starter cultures in cheese and fermented dairy products. Morphological, biochemical and physiological features of the selected isolates were performed in their identification. A total of 226 isolates were recovered from 40 yogurt samples (23.45%), 30 Ras chesse samples (19.47%), 48 Kariesh cheese samples (33.18%) and 32 samples of Laban Rayeb (23.9%) that were randomly collected from local markets in Dakahlia governorate. The distribution of LAB isolates by the genus was as follows: Lactobacillus (33.63%), Streptococcus (17.69%), Lactococcus (8.85%) and Enterococcus (39.82%). Among these isolates, E. faecalis (55 isolates, of 24,34%), S. thermophilus (40 isolates, of 17.69%), Lb.delbrueckii subsp. bulgaricus (30 isolates, of 13.27%) E. faecium (25 isolates, of  11.06%), Lb. helveticus (16 isolates, of  7.1%), Lb. salivarius (15 isolates, of  6.64%), Lc.lactis subsp. lactis (15 isolates, of 6.64%), Lb. acidophilus (10 isolates, of 4.42%), E. gallinarum (7 isolates, of 3.1%), Lc. Lactis subsp. cremoris (5 isolates, of  2.21%) and E. malodoratus (3 isolates, of 1.33%). These 226 isolates were further examined for their capability to produce antimicrobial compounds.  Only 16 out of 226 LAB isolates could inhibit the growth of the examined indicator organisms. Among these isolates, 8 LAB isolates (3 isolates of Enterococcus fecalis, 2 isolate of each E.faecium, E. gallinarum and E. malodoratus ) were still capable to inhibit the indicator organisms by neutralization to pH 6.8. These isolates may produce bacteriocin like substances.   

DOI

10.21608/jfds.2018.35866

Keywords

lactic acid bacteria, isolation, Dairy products, Antimicrobial

Authors

First Name

T.

Last Name

Nassib

MiddleName

A.

Affiliation

Dairy Department, Faculty of Agriculture, Mansoura University

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City

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Orcid

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First Name

M.

Last Name

Darwish

MiddleName

S.

Affiliation

Dairy Department, Faculty of Agriculture, Mansoura University

Email

-

City

-

Orcid

-

First Name

Ola

Last Name

Shalabi

MiddleName

M. A. K.

Affiliation

Dairy Department, Faculty of Agriculture, Mansoura University

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-

City

-

Orcid

-

Volume

9

Article Issue

6

Related Issue

5883

Issue Date

2018-06-01

Receive Date

2018-05-24

Publish Date

2018-06-03

Page Start

193

Page End

199

Print ISSN

2090-3650

Online ISSN

2090-3731

Link

https://jfds.journals.ekb.eg/article_35866.html

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https://jfds.journals.ekb.eg/service?article_code=35866

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3

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Original Article

Type Code

886

Publication Type

Journal

Publication Title

Journal of Food and Dairy Sciences

Publication Link

https://jfds.journals.ekb.eg/

MainTitle

Isolation and Identification of Antimicrobial Producing Lactic Acid Bacteria from Local Egyptian Dairy Products

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Article

Created At

22 Jan 2023