The present study was carried out on both marine and fresh water edible crustaceans namely; Erugosquilla massavensis and Procambarus clarkii, respectively, which are predominant in the local aquatic fauna in Egypt. The present study was conducted to determine the total antioxidant capacity (TAC), total phenolic, total flavonoid and carotenoid content in muscle and exoskeleton of E. massavensis and P. clarkii crustaceans. The study also evaluate the antioxidant, antimicrobial and antitumor activities of the carotenoid extracted from these species. The results showed that TAC, phenolic and flavonoid contents in both muscle and exoskeleton of E. massavensis are higher than those contents in P. clarkii. The results showed also that the carotenoid content extracted from the males and females exoskeleton of E. massavensis are higher than those of males and females of P. clarkii. The antioxidant activity of carotenoid extracted from male and female of P. clarkii showed higher activity than those extracted from E. massavensis. By contrast, carotenoids extracted from females and males of E. massavensis showed higher antimicrobial activity than those of females and males P. clarkii. The carotenoid extract shows relatively weak antitumor activity in vitro against the Earlich cell lines. In conclusion, both of E. massavensis and P. clarkii have a bioactive compound with antimicrobial and antioxidant activities.