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259830

THE USE OF BANANA PEEL FOR CELLULOLYTIC ENZYMES PRODUCTION BY A LOCAL STRAIN OF Aspergillus niger

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Last updated: 04 Jan 2025

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Abstract

Banana peel was used as a carbon source to produce cellulases and cellobiase in shake flask cultures by A. niger.  Peak cellulases and cellobiase activities were obtained on the 6th day of fermentation.  The temperature and pH influenced the yield of enzymes.  The maximum cellulase activities were recorded at 30°C and pH 5.0.  The maximum rate of enzyme activities was obtained when A. niger was grown on 1.5% banana peel.  Deprivation of ammonium sulphate, peptone or nitrogen source as whole inhibited cellulolytic enzyme formation.  The maximum enzyme activities were recorded at 40°C except cellobiase, which had maximum activity at 30°C.  The optimum pH of CMCase, FPase and cellobiase was 5.5, 6.5 and 5.5, respectively.  The maximum CMCase and cellobiase activities were detected after 1 h, whereas the maximum FPase was detected after 16 h.  More than 50% of CMCase, FP-activity and cellobiase retained at 60, 70 and 60°C, respectively.

DOI

10.21608/jacb.2000.259830

Keywords

banana peel, cellulolytic enzymes, Aspergillus niger, Carboxymethyl cellulase, filter paper-ase, cellobiase

Authors

First Name

F.

Last Name

Hauka,

MiddleName

I. A.

Affiliation

Agric. Microbiol. Dept., Fac. of Agric., Mansoura Univ, Mansoura, Egypt.

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First Name

A.

Last Name

Selim

MiddleName

E. I.

Affiliation

Agric. Microbiol. Dept., Fac. of Agric., Mansoura Univ, Mansoura, Egypt.

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City

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Orcid

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First Name

Samia

Last Name

Bayoumy

MiddleName

M. M.

Affiliation

Agric. Microbiol. Dept., Fac. of Agric., Mansoura Univ, Mansoura, Egypt.

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Volume

25

Article Issue

10

Related Issue

36739

Issue Date

2000-10-01

Receive Date

2000-09-25

Publish Date

2000-10-01

Page Start

6,503

Page End

6,512

Print ISSN

2090-3626

Online ISSN

2090-3707

Link

https://jacb.journals.ekb.eg/article_259830.html

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https://jacb.journals.ekb.eg/service?article_code=259830

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7

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Original Article

Type Code

883

Publication Type

Journal

Publication Title

Journal of Agricultural Chemistry and Biotechnology

Publication Link

https://jacb.journals.ekb.eg/

MainTitle

THE USE OF BANANA PEEL FOR CELLULOLYTIC ENZYMES PRODUCTION BY A LOCAL STRAIN OF Aspergillus niger

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Article

Created At

22 Jan 2023