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255926

USAGE OF ELECTROMAGNETIC FIELD AS A TOOL FOR DENATURATION OF GENOMIC DNA FROM Saccharomyces cerevisiae.

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Last updated: 04 Jan 2025

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Abstract

This study reports the effect of the energy of magnetic fields for usage as a
denaturing agent of genetic material (DNA) from Saccharomyces.cerevisiae. Two
different flux densities (0.1 and 0.5 T units) of magnetic field were applied to two
different haploid yeast strains (Ura 1 and Gm3) for both 5 minutes and 15 minutes.
The absorption spectra of DNA exposed to the magnetic field was analyzed at
230nm,260 nm, and 280 nm, at two different temperatures (35°C and 72"C).
Absorbance spectra thus generated was compared to corresponding spectra obtained
from S.cerevisiae DNA ,not exposed to magnetic field. At 720C absorbance was
much higher in DNA samples exposed to magnetic field after their purification. Much
higher impact of magnetic field was observed in the strain having more mutants. The
magnetic field increased the energy of hydrogen bonds separated or break its and
hard DNA double helix (denaturation). This effect depends on the genetic background.

DOI

10.21608/jacb.2002.255926

Keywords

Electromagnetic Field, yeast, DNA denaturation

Authors

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S.

Last Name

Sharaf EI-Deen,

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Affiliation

Microbial Genetics Department, National Research Centre, Cairo, Egypt.

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Volume

27

Article Issue

7

Related Issue

36317

Issue Date

2002-07-01

Receive Date

2002-06-15

Publish Date

2002-07-01

Page Start

4,923

Page End

4,933

Print ISSN

2090-3626

Online ISSN

2090-3707

Link

https://jacb.journals.ekb.eg/article_255926.html

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https://jacb.journals.ekb.eg/service?article_code=255926

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Original Article

Type Code

883

Publication Type

Journal

Publication Title

Journal of Agricultural Chemistry and Biotechnology

Publication Link

https://jacb.journals.ekb.eg/

MainTitle

USAGE OF ELECTROMAGNETIC FIELD AS A TOOL FOR DENATURATION OF GENOMIC DNA FROM Saccharomyces cerevisiae.

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Article

Created At

22 Jan 2023