Some cyanobacteria isolates were collected from soil samples at various locations from governorates of Kafr El-Sheikh and El-Dakahlia. By using morphological characterizations to identify these isolates. Heterocyst-forming cyanobacteria were Anabaena sp., Nostoc sp., Oscillatoria sp. and Chroococcus sp. Only, Nostoc sp.and Anabaena sp < em>. isolates were represented at high frequency in the two isolated areas. The less frequency of occurrence in this study were Oscillatoria sp., Chroococcus sp., Phormidium sp., Pseudoanabaena sp. Nostoc calicola D and Anabaena cylindrica D were recorded as the most active cyanobacteria according to nitrogen fixation, nitrogenase activity, Indole Acetic Acid(IAA) production and dry weight determination. Therefore, it would be recommended to apply these strains of cyanobacteria in bio-organic farming.