Grape seed oil is rich in phenolic compounds, fatty acids and vitamins which have high economic, and importance. It is used as an edible oil due to its beneficial properties for health, that are detected by many studies, such as anticancer, cardio protective and protective effect against cellular toxicity, induced by the environmental pollutants such as carbon tetrachloride (CCL₄) . This pollutants generates free radical may cause a damage of the liver which is responsible for the metabolism of drugs and chemical toxic. The aim of this study was to detect the chemical composition of oil extracted from grape seeds (medium solvent) and antioxidant potential of oil by mixing with milk by product namely skim milk. Include the ameliorative effect against oxidative damage in cells induced by CCL₄. The results of revealed that the extracted grape seed oil is rich in unsaturated fatty acids and low concentrations of saturated ones. Also, the resulting oil has a high antioxidant capacity incorporate with the product namely skim milk. In addition the incorporate of oil with skim milk has a prophylactic effect against the toxicity induced in liver by CCL₄. On the other hand, enhance the activity of antioxidant enzymes namely Glutathione and Glutathione-S-Transferees. And antioxidant prosperity is claimed by a food model to be one of the mechanism of natural and hepato protective antioxidant.