Four inulinolytic yeast isolates were isolated and identified by molecular methods. They were belonged to Candida catenulata, Sarocladium kiliense, Galactomyces candidum and Scopulariopsis brevicaulis. The production of inulinase by the previous isolates through, 72h was done on two media using jerusalem artichoke tubers, garlic bulbs, dahlia tubers and chicory roots as natural sources of inulin. The highest values of the produced inulinase were obtained on Bharathi et al. (2011) medium after 48h of incubation. Jerusalem artichoke proved to be the best inulin source for enzyme production by Scopulariopsis brevicaulis (38.22 µg/ml), followed by dahlia (32.45 µg/ml). The best substrate for inulinase production by Sarocladium kiliense was dahlia (29.21 µg/ml) followed by garlic (28.65 µg/ml).  As for Galactomyces candidum the maximum enzyme production was obtained using jerusalem artichoke (34.96 µg/ml) followed by garlic (31.84 µg/ml). The best material for inulinase production by Candida catenulate (34.15 µg/ml) was dahlia, followed by garlic (31.40 µg/ml). It can be concluded that inulin containing plant materials could be employed as a carbon source for inulinase production, it offer advantage in comparison to purified substrate because it have low cost and high productivity.