Random amplified polymorphic DNA (RAPD) primers associated with drought tolerance was used in this study to characterize drought tolerance in six wheat genotypes with developed marker assisted drought tolerance.Four of them were tolerant and two were drought sensitive genotypes..The results indicated that tolerant genotypes  were harboring seven positive RAPD markers ,while sensitive genotypes were having only one negative RAPD markers..In tolerant genotypes, seven positive PCR-RAPD markers with molecular sizes of 1050bp, 390 bp, 200bp.230bp ,850bp, 430bp and 800bp were exhibited by A-12 ,B-05,C-12 ,E-10 and B-02 primers. This study indicated that the seven positive markers can be used as indicators to discard drought tolerance in wheat marker -assisted breeding programes.