The contamination of animal feed with mycotoxigenic fungi continues to be a disastrous problem along the food chain. Pichia anomala was applied as a biocontrol yeast to prevent Aspergillus flavus growth in vitro and in feed corn. The yeast strain able to inhibit A. flavus growth and the enzymes assay indicated that P. anomala was able to produce β-1,3-glucanase and chitinase which could propose a mode of action for its antifungal activity. Scanning electron micrographs, of fungal hypha cultures with P. anomala, revealed that yeast cell could colonize the fungal hypha leading to their lysis and deterioration. The application of P. anomala as a protein supplement resulted in apparent increase in protein content in feed grain and animals' weight gain, in addition to a remarkable decrease in the mortality rate among fed animals after consumption of P. anomala -supplemented feed.