Two lizemeters experiments in of Bacteriology Lab., Sakha Agricultural Research Station were undertaken to evaluate the effect of biofertilization and organic farming systems on yield components and rhizosphere bacterial counts of wheat plant, cultivars (Misr 1 & Seds 12 through winter seasons of 2013 and 2014). The results indicated that grains yield and weight of 100 grains of wheat cultivars significantly increased due to biofertilization system, while there were no significant differences in grains yield due to application of the organic farming system application. In contrast, organic farming treatment achieved significant increases in weight of 100 grains as compared with traditional control treatment. In respect to protein percentage of wheat grains, biofertilization treatment caused increases over traditional one, while, organic farming system attained significant decreases than those of traditional control, and this decreases were clear for Sids 12 than Misr 1 cultivar. Mineral concentrations in wheat grains showed different trends regarding their response to biofertilization and organic farming systems. N percentage did not significantly affected by biofertilization; however, there were significant decreases due to application of organic farming system than that of traditional control. Regarding phosphorous concentration, showed different response trends, whereas, biofertilization and organic farming treatments increased wheat grains of both cultivars at the two seasons, while the differences were significant under organic farming treatment only. The studied systems over traditional control, whoever, the differences did not reach to the level of significance. Total bacterial counts of the rhizosphere soil of wheat plants increased due to biofertilization and organic farming treatments over those for traditional one, the increases were significant. The highest recorded counts achieved in case of organic farming treatment. Otherwise the counts of Azospirillum sp. achieved the same trend, but the increases due to biofertilization and organic treatments over that of traditional control were highly significant.