Extracted from ginger rhizome (Zingiber officinale L.)  with boiling water crude polysaccharides. polysaccharides extracted was separated into four fractions through chromatographic analysis on DEAE-cellulose and Sephadex G-200 column, GP1, GP2, GP3 and GP4 were separated. It has been identified at the molecular weight of polysaccharides (GP1, GP2, GP3 and GP4) separated by The gel permeation chromatography (GPC) analysis were approximately 136, 27.7, 11.8 and 11.4 kDa, respectively. Using a GC-MS analysis was to identify the monosaccharides analysis, the results showed the presence of monosaccharides following (mannose, glucose and galactose) larger amounts of monosaccharides (rhamnose, xylose and fructose), I found that the amount is less compared to other monosaccharides. An evaluation of antioxidant activity that GP4 compound was good potential for scavenging activity of DPPH radical, ABTS radical and higher scavenging activity of hydroxyl radicals compared to other studied polysaccharide, should be explored as antioxidants in the new studies.