The research was carried out for isolation of amylase producing bacteria from various starchy locations.  Thirty one bacterial isolates were screened in order to select the best amylase producing and identified as Bacillus subtilis. Optimization environmental condition factors were studied as ;organic nitrogen sources (beef extract, peptone, yeast extract, urea, or inorganic as; ammonium chloride, ammonium sulfate and sodium nitrate).Also carbon sources (sucrose, anhydrous dextrose,    D+ glucose, lactose, corn starch, maltose, soluble starch and potato starch) were studied. The highest yield of amylase production by B. subtilis were achieved in cultures supplemented with sodium nitrate was; 1.34±0.032^c U/ml/min. on the other hand, maltose gave a high yield of amylase production 0.511±0.046 U/ml/min as carbon source.  The optimum pH was 9.0 which gave 1.49±0.055 U/ml/min, while temperature 30c^owas better which gave 0.544±0.015 U/ml/min.  Maximum amylase production was obtained by application of cheaper agro-industrial wastes type as carbon source, the highest yield was obtained with banana peels which gave; 0.638±0.004 U/ml/min.