Egyptian olive pomace was collected from Presses Salehia Oils (Salhia, Sharkia, Egypt).The investigation was performed to study the composition (moisture, crude fat, crude protein, carbohydrate, fiber and total ash) of olive pomace. The qualitative phytochemical analysis stated the presence of flavonoid, tannins, and alkaloids, in acetone and methanolic70% extracts of olive pomace. Saponin and reducing sugar were found in methanol extract but not found in acetone extract. Starch, monosaccharide, reducing sugar and anthraquinone glycosides were not found in acetone and methanol extracts. Terpenoidswere found in acetone extract and not found in the methanol extract. Results stated that the phytochemicals recovery dependedupon the polarity of the used solvent. Total flavonoids and total polyphenols were determined. Glycones, aglycones and simple phenolic compounds in olive pomace acetone and methanol extracts were detected by HPLC. Antioxidant activity, antibacterial properties against gram-positive and gram-negative bacteria as well as fungi and anticancer activity of olive pomace extracts were studied. Olive pomace was found to possess a very potent inhibitory effect against hepatocellular carcinoma (HePG2)cell line, breast carcinoma (MCF7 and T47D)cells lines and colon carcinoma (HCT116) cell line.