One hundred and five Mandara laying hens, 24 wks of age, were divided into seven experimental treatments to study the effect of dietary levels of fish oil (FO), linseed oil (LO) and their combination as a source of omega 3 on egg yolk omega three polyunsaturated fatty acids concentration on cholesterol and total lipids, and the productive performance and egg quality of laying hens. The experimental treatments were divided into seven treatments with three replicates each. The first treatment group was the control group, the second treatment was given 1% FO, the third treatment was given 2% FO, the fourth treatment was given2% LO, the fifth treatment was given 3% LO, the sixth treatment was given 1% FO + 1% LO and the seventh treatment was given1.5% FO + 1.5% LO. The obtained data revealed the following results: Addition of FO, LO, and LO + FO to the diets did not cause significant effects (P > 0.05) on feed intake, feed conversion and egg production traits (egg number, egg weight and egg mass). Adding FO, LO, and their combination insignificantly increased yolk percentage.Saturated fatty acids significantly decreased FO and LO increased compared to the control. Diets containing FO, LO, and their combinations resulted in a significant increase of n-3 polyunsaturated fatty acids and polyunsaturated fatty acid concentrations in eggs compared to the control group.Adding 2% FO, 1% FO + 1% LO and 1.5% FO + 1.5% LO to laying diets decreased (P<0.05) egg yolk total cholesterol and egg yolk total lipids comparing to control group. The lowest values of egg yolk total cholesterol and egg yolk total lipids were recorded by 1.5% FO + 1.5% LO compared to other treatments. Using 1% FO only or 1% FO + 1% LO in laying hen diets improved the economical efficiency comparable to the control group. The best feed cost/kg egg was recorded by the group fed diet 1% FO + 1% LO.