A trial was conducted on Nile tilapia Oreochromis niloticus with intial weight 0.83 ± 0.04g/fish, for 12 weeks in aquatic lab of department of Animal and Fish Production in the College of Agricultural and Food Sciences, to investigate the effects of black seed Nigella sativa on feed intake , growth, and general performance. Five balanced diets were prepared (equal in protein and energy content) at various concentrations of black seed (0, 0, 5, 10 and 20g/kg), and stored at similar conditions that corresponding to prevailing enviromental conditions of kingdom of Saudi Arabia fish farms, particularly those exposed to temperature, Humidity and direct sunlight for 4 months. However, dueuar suwk of (0g) was preserved at 4C^o as standard until used. Feeding of fish was three times a day until satiation. The results of peroxide analysis of diets demonstrated that the oxidation of feed components (free of black seed) which stored in prevailing conditions of Saudi ongoing fish farms exceeded the acceptable level in feed with amount of 17.63 ml equivalent/kg, while other feeds were remained within acceptable levels. On the other hand, results of the study showed significant differences between control treatment and other treatments, in term of final weight, gained weight, Specific growth rate, consumed feed, feed conversion ratio, protein efficiency yatioand protein productive value. Regarding the survival rate, the results showed that no significant differences between the control treatment fish and other treatments except with second treatmen (free of black seed) which survival rate was reduced to 30%. Results affirmed no significant differences between control treatment and other treatments which contained black seed at concentrations 5g, 10g and 20g 1kg  diet, in term of crude protein and ether extract; while there were significant differences between control treatment and second treatment (free black seed), and between the second treatment and other treatments in crude protein and ether extract. In addition, there was a significant difference in the content of fish moisture between control treatmen and other treatments. Also, there were no significant differences between control treatment and other treatments in ash content.