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81016

GENETIC DIVERSITY AND RELATIONSHIPS AMONG GRAPEVINE ROOTSTOCK MUTANTS THROUGH RAPD TECHNIQUE

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Last updated: 04 Jan 2025

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Abstract

Mutation using radiation has been shown to be an important tool in incorporating specific desirable agronomic value characteristics. In the present study, four grapevine rootstocks namely: Freedom, Harmony, SO4 and Ramsey   along with commercial variety Thompson seedless were exposed to different doses of gamma rays (0, 10, 20 Gy) to obtain mutations with economic values. Results showed that both doses of 10 and 20 Gy were effective in obtaining mutations in all grapevine genotypes including Thompson seedless, which were confirmed by morphological markers such as bud burst percentage and shoot length and number of leaves, and genetic markers by RAPD technique. In addition, genetic variation and relationships in both irradiated and un-irradiated genotypes were determined. Six RAPD markers were able to generate polymorphic bands ranged from 1 to 9 among irradiated and un-irradiated grapevine genotypes. The similarity coefficients detected by RAPD markers ranged from 0.24 to 0.87, which revealed clear genetic variation among irradiated and un-irradiated grapevine genotypes. According to phylogenetic analysis, genotypes were divided into two clusters, where all five un-irradiated genotypes and irradiated Freedom genotype with 10 Gy were placed in one cluster and second cluster included the rest of nine irradiated genotypes.  Conclusively, this study supports the use of mutation in breeding grapevine improvement, and RAPD technique could be an important tool for detecting mutations and genetic diversity among grapevine genotypes.    

DOI

10.21608/jpd.2020.81016

Keywords

Mutation, grapevine varieties, RAPD technique, rootstocks

Authors

First Name

Asmaa

Last Name

Salama

MiddleName

Mohi

Affiliation

Viticulture Research Department, Horticulture Res. Inst., Agric. Res. Center, Giza, Egypt

Email

asmaa_mohi@yahoo.com

City

Horticulture Res. Inst., Agric. Res. Center

Orcid

-

First Name

Mohamed

Last Name

Zayan

MiddleName

-

Affiliation

Pomology Department, Faculty of Agriculture, Kafrelsheikh University, Egypt

Email

-

City

Faculty of Agriculture, Kafrelsheikh University

Orcid

-

First Name

Aly

Last Name

Shereif

MiddleName

-

Affiliation

Pomology Department, Faculty of Agriculture, Kafrelsheikh University, Egypt.

Email

-

City

Faculty of Agriculture, Kafrelsheikh University

Orcid

-

First Name

Aisha

Last Name

Gaser

MiddleName

-

Affiliation

Pomology Department, Faculty of Agriculture, Kafrelsheikh University, Egypt.

Email

-

City

Faculty of Agriculture, Kafrelsheikh University

Orcid

-

Volume

25

Article Issue

1

Related Issue

12173

Issue Date

2020-01-01

Receive Date

2019-11-15

Publish Date

2020-01-01

Page Start

79

Page End

99

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 25_Issue 1_Pages 79-99.pdf

PDF . 694.4kB

Link

https://jpd.journals.ekb.eg/article_81016.html

Detail API

https://jpd.journals.ekb.eg/service?article_code=81016

Order

5

Type

Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

MainTitle

GENETIC DIVERSITY AND RELATIONSHIPS AMONG GRAPEVINE ROOTSTOCK MUTANTS THROUGH RAPD TECHNIQUE

Details

Type

Article

Created At

22 Jan 2023