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42552

COMPARATIVE STUDIES ON Catharanthus sp. CULTIVARS USING CHLOROPHYLL CONTENT AND PEROXIDASE ISOZYME

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Last updated: 22 Jan 2023

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Abstract

Three Egyptian Catharanthus cultivars belong to family apocyanaceae {Roseus, Ocellatus and Albus} as well as calli were derived from leaf explant for each and grown on MS supplemented with 1mg/l NAA and 1 mg/l BA,  were studied using chlorophyll contents and peroxidase isozyme  . According to the contents of chlorophyll a, b and the total chlorophyll, Roseus, and Ocellatus cultivars' chlorophyll contents were more closely related to each other, but distantly related to Albus cultivar. The total chlorophyll contents were 22.86, 22.08 and 30.51 for Roseus and Ocellatus and Albus cultivars, respectively. Peroxidase isozyme study, exhibited five bands in shoots of the three cultivars, Two bands migrated towards the anode (Pex-A1 and Pex-A2) while the other three bands migrated towards the cathode (Pex-C1, Pex-C2 and Pex-C3). Peroxidase isozyme in the callus samples showed the absence of Pex A1 and Pex A2 bands in Ocellatus cultivar, and the absence of Pex A1, Pex A2 and Pex C3 in Albus cultivar but not in Roseus one where the all 5 bands were exist .No differences between cultivars were resulted when shoot samples. Conclusively, it could be concluded that chlorophyll contents and isozyme analysis could be considered as a useful tool for cultivar identification because it is reliable, rapid and can provide identification at some stages in plant life cycle. Isozyme technique can be used to identify the desired genotypes and to exclude the cultivars before establishing; it may also serve to identify the more active cultivar, which is essential for biotechnological programmes.          

DOI

10.21608/jpd.2013.42552

Keywords

Catharanthus, peroxidase, isozymes, Chlorophyll

Authors

First Name

Ibrahim

Last Name

Ibrahim

MiddleName

-

Affiliation

Plant Biotechnology Department, Genetic Engineering and Biotechnology ,Research Institute (GEBRI), Sadat City, Minufiya University , Egypt.

Email

mostaw100@yahoo.com

City

elsadat city

Orcid

-

First Name

Alaa El din

Last Name

Hemeida

MiddleName

-

Affiliation

Bioinformatic Department, Genetic Engineering and Biotechnology, Research Institute Minufiya University, Egypt.

Email

-

City

Sadat city

Orcid

-

First Name

Ama

Last Name

.Hussain

MiddleName

-

Affiliation

Bioinformatic Department, Genetic Engineering and Biotechnology, Research Institute Minufiya University, Egypt.

Email

-

City

Sadat city

Orcid

-

First Name

Metwally

Last Name

Bekhit

MiddleName

-

Affiliation

Plant Biotechnology Department, Genetic Engineering and Biotechnology ,Research Institute (GEBRI), Sadat City, Minufiya University , Egypt.

Email

-

City

Sadat city

Orcid

-

First Name

Amal

Last Name

Zweil

MiddleName

-

Affiliation

Plant Biotechnology Department, Genetic Engineering and Biotechnology ,Research Institute (GEBRI), Sadat City, Minufiya University , Egypt.

Email

-

City

Sadat city

Orcid

-

Volume

18

Article Issue

1

Related Issue

6671

Issue Date

2013-01-01

Receive Date

2012-11-09

Publish Date

2013-01-01

Page Start

77

Page End

84

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 18_Issue 1_Pages 77-84.pdf

PDF . 449.1kB

Link

https://jpd.journals.ekb.eg/article_42552.html

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https://jpd.journals.ekb.eg/service?article_code=42552

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7

Type

Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

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Article

Created At

22 Jan 2023