Objective: To assess effect of different bite recording materials on accurate mounting of working casts. Materials and Methods: A 40 maxillary and mandibular stone casts were obtained from dentoform teeth using polyether impression material. Derived stone casts were divided according to bite registration material in to four groups (10 samples each); 1. wax group, 2. polyvinyl siloxane group, 3. polyether group and 4. Bis-crylic resin group. Ten bite records were obtained with poly vinyl siloxane recording material from hand articulated dentoform to serve as control group. The 40 produced mandibular stone casts were mounted on semi adjustable articulators using four bite registrations tested materials. Unilateral Polyvinyl siloxane bite registration were made for 40 mounted stone casts to evaluate bite registration accuracy. Then bite records were placed on a light box and a camera set at a fixed distance 8 inches was used to capture the light transmission that was projected through the bite material. The camera transferred the information to an image analysis program (ImageJ) which measure accuracy of registration materials. Results: Grey scale data showed parametric distribution while measurement error showed non-parametric distribution. The difference between tested groups and control group was compared using ANOVA test. The results revealed that, the different between test groups and control was insignificant (P-value = 0.104). Conclusions: All tested bite registration materials had comparable results, although Luxabite and polyether showed higher errors than polyvinyl siloxane and wax.