This study was designed to investigate the effect of some factors affecting on in vitro propagation of six canola genotypes (Serw-6, Pactol, Wan-you 25, RG-4514, Serw-4 and AD-201/Gi/ 51). During establishment stage, different medium types (MS and B5) and cytokinin types (BA, Kin and 2ip) were investigated. MS medium supplemented with BA at 1.0 mgl^-1 proved to be the best treatment for all tested genotypes during this stage. Also, different explant types (shoot tip, internode and hypocotyl), BA concentrations (0.0, 1.0, 3.0 and 5.0 mg l^-1) were examined during multiplication stage. Results indicated that while explant type had no effect on shoot induction and growth, BA proved to be the best cytokinin especially at 1.0 mgl^-1 since it produced the maximum number of shoots/ explant for the most tested genotypes. During rooting stage, effects of MS medium strength (full and half) and auxin type (IAA, IBA and NAA) at the concentration of 1.0 mgl^-1 were evaluated. Generally, using of full MS medium supplemented with 1.0 mgl^-1 IBA stated to be the most suitable treatment for root induction and growth of the most investigated genotypes. Obtained plantlets were successfully acclimatized (60-80% survivability) in peat moss and sand (1:1, V/V) medium in the greenhouse.