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49860

CONTAMINATION OF FERMENTED FOODS IN EGYPT WITH UNDESIRABLE BACTERIA

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Last updated: 30 Jan 2023

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Abstract

This study assessed the safety and quality of some fermented foods in Egypt. The fermented milk (yogurt), sour milk (Laban Rayeb) and flour fermented barley (Buza or Boza) samples were collected from Sharkia Governorate (Zagazig, Faqous, Abu Kebir and El Husseiniya District), Egypt. Total bacterial diversity, lactic acid bacteria, yeasts and molds, staphylococci and micrococci, coliforms, Enterobacteriaceae, pseudomonads and enterococci counts within each sample were determined. The identity of the isolates was established through microscopic, biochemical and molecular techniques. The total bacterial counts and lactic acid bacteria of analysed samples ranged from 6.70 to 9.23 log cfu/ml and 6.09 to 9.24 log cfu/ml, respectively. The overall results on the bacterial biota of the fermented foods revealed that total bacterial loads of yogurt, Laban Rayeb and Boza were 8.30, 9.23 and 8.19 log cfu/ml, respectively. Generally, results revealed that undesirable bacterial isolates from de Man, Rogosa, and Sharpe agar (MRS) and M17 selective media for lactic acid bacteria belonged to Bacillus, Pseudomonas and Staphylococcus genera and fungal isolates belonged to Saccharomyces and Candida. genera. The most dominant bacterial species identified by 16S rRNA sequencing analysis were B. sporothermodurans, B. thuringiensis, B. safensis, B. sphaericus B. tequilens, B. licheniformis, B. vietnamensis, B. amyloliquefaciens, B. subtilis,  P. psychrotolerans, S. cohnii and S. saprophyticus. This study proved the presence of unusual bacteria contaminants (UBC) in the selected fermented foods. The UBSs could be pathogenic and/or toxigenic by producing various types of toxins.

DOI

10.21608/zjar.2018.49860

Keywords

Yogurt, Laban Rayeb, buza, unusual bacteria contaminants, Bacillus

Authors

First Name

Safaa

Last Name

Omar

MiddleName

M.

Affiliation

Agric. Microbiol. Dept., Fac. Agric., Zagazig Univ., Egypt

Email

samahgoub@zu.edu.eg

City

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Orcid

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First Name

H.

Last Name

Abd El-Fattah

MiddleName

I.

Affiliation

Agric. Microbiol. Dept., Fac. Agric., Zagazig Univ., Egypt

Email

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City

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Orcid

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First Name

Howaida

Last Name

Abdelbasit

MiddleName

M.

Affiliation

Agric. Microbiol. Dept., Fac. Agric., Zagazig Univ., Egypt

Email

-

City

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Orcid

-

First Name

S.

Last Name

Mahgoub

MiddleName

A.

Affiliation

Agric. Microbiol. Dept., Fac. Agric., Zagazig Univ., Egypt

Email

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City

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Orcid

-

Volume

45

Article Issue

1

Related Issue

7704

Issue Date

2018-01-01

Receive Date

2017-11-20

Publish Date

2018-01-01

Page Start

317

Page End

329

Print ISSN

1110-0338

Link

https://zjar.journals.ekb.eg/article_49860.html

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https://zjar.journals.ekb.eg/service?article_code=49860

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Original Article

Type Code

842

Publication Type

Journal

Publication Title

Zagazig Journal of Agricultural Research

Publication Link

https://zjar.journals.ekb.eg/

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Article

Created At

22 Jan 2023