This study was carried out at Plant Tissue Culture Laboratory, Faculty of Environmental
Agricultural Sciences, El-Arish University, North Sinai Governorate, Egypt during 2014 to 2017.
Effect of three medium types, Murashige and Skoog (MS), Linsmaier and Skoog (LS) and Lloyd and
McCown (WPM), two explant types (shoot-tip and single node cutting), three cytokinin types
(Benzyladenine, Kinetin and 2-isopenteny1 adenine), two additives; viz., s (thiamine and pyridoxine
hydrochloride) and amino acids (asparagine and arginine) and two gelling agents (guar seed powder
and locust bean seed powder) combined with agar on micropropagation of Haplophyllum tuberculatum
were investigated. For rooting, the effect of three auxins (Indole acetic acid, Indol butyric acid and
Naphthalene acetic acid) were studied in combination with three medium strengths (full, half and
quarter) combined with the best mixture (0.50 mgl-1 Kin + 6 gl-1 agar with 2 gl-1guar seed powder) of
gelling agents. Obtained results showed that Murashige and Skoog (MS) was the best medium with
single node explant as starting material during establishment stage. Multiple shoots were obtained on
MS medium supplemented with 1.0 mgl-1 Kinetin (Kin) combined with 0.1 mgl-1 α-naphthalene acetic
acid (NAA). Thiamine and arginine were the best vitamins and amino acid at 0.1 mgl-1 and 0.50 mgl-1,
respectively. Agar at 6 gl-1 + guar seed powder at 2 gl-1 was the best gelling agent treatment. Half
strength MS medium with 0.50 mgl-1 Kin + 0.5 mgl-1 NAA and 6 gml-1 agar with 2 gml-1 guar seed
powder was the best combination for rooting were successfully (85% survival) acclimatized in mixture
of peatmoss, vermiculite, washed sterilized sand and perlite (1:1:1:1, V/V/V/V).