This experiment amid to investigate the effect of two strawberry cultivars (Festival
and Sweet Charlie), two auxins, i.e. Indole-3-butyric acid (IBA) and1-Naphthalene acetic acid (NAA)
and activated charcoal on the in vitro roots formation (rooting stage) of strawberry plantlets. The
obtained results showed that, Sweet Charlie cultivar being the superior one and recorded the maximum
values of both root and shoot length, number of roots and leaves, the fresh and dry weight of roots and
leaves of plantlet as compared with Festival cultivar. It is evident that, using ½ MS-medium
supplemented with 1.0 mg /l IBA+0.5 g /l activated charcoal being the most effective and superior
treatment for increasing both root and shoot length. While, rooting medium contained ½ MS-medium
+ 1 .0 mg /l IBA or 0.5mg /l IBA +0.5 mg /l NAA recorded the highest values of number of both roots
and leaves per plantlet .In addition ,using ½ MS-medium supplemented with 1.0 mg /l NAA recorded
the maximum values of the fresh and dry weight of roots per plantlet. Furthermore, the highest value
for each of fresh weight of crown and leaves per plantlet, was obtained by using ½ MS-medium
supplemented with 0.5 mg /l IBA+0.5 mg /l NAA. On the other hand, using ½ MS–medium + 0.5 mg
/l IBA + 0.5 mg /l NAA + 0.5g /l activated charcoal being the superior treatment and recorded the
maximum values of nitrogen, phosphorus, potassium, total carbohydrate and total protein content (%).
Transfer shoots of Sweet Charlie cultivar to the rooting medium contained ½ MS- medium +1.0 mg /l
IBA +0.5 g/l activated charcoal recorded the maximum values of both root and shoot length, number
of leaves per plantlet, the fresh and dry weight of leaves, as well as the dry weight of crown per
plantlet. While, the highest number of roots per plantlet was more achieve via using ½ MS-medium +
1.0 mg/l IBA. Moreover, cultured shoots of Festival cultivar on ½ MS-medium supplemented with 0.5
mg /l IBA+0.5 mg /l NAA being the most effective and favorable treatment for increasing the fresh
weight of roots per plantlet. On the other hand, the highest increase of the fresh weight of crown per
plantlet were more distinct via the interaction treatment between such cultivar and using ½ MSmedium
supplemented with 1.0 mg/l NAA. Furthermore, using the rooting culture medium contained
½ MS- medium+0.5 mg/l IBA+0.5 g /l activated charcoal recorded the highest value for each of
nitrogen and total protein content (%) in the plantlets of Festival cultivar, while cultured shoots of
Sweet Charlie cultivar on the same rooting medium, registered the maximum increase of phosphorus,
potassium and total carbohydrates content (%).