A virus causing different symptoms of mosaic, mottling, leaf rolling and distortion in (Vicia faba) was found in various fields in Menofia Governorate, Egypt. The virus isolate was detected and identified to bean yellow mosaic virus (BYMV) using symptomatology, indicator plants, serological tests (DAS-ELISA and Dot blot immunoassay (DBIA)) and Light microscope. Also, the study conduct to evaluate the efficiency of two strain of Rhizobium leguminosarum (R.  leguminosarum strain MNF-EM-R4 and R.  leguminosarum bv.viciae strain MNF-EM-R9) for  inducing systemic acquired resistance in faba bean to bean yellow mosaic virus (BYMV).Our findings showed that  grown faba bean plants from treated seeds with rhizobia and inoculated with BYMV presented a imperative reduction in disease occurrence percentage and ELISA reads. While, these treatments caused an increase inPeroxidase enzyme activity compared with control. SDS-PAGE analysis showed that treatment with R. leguminosarum strain MNF-EM-R4 showed one band, this band did not appear in negative control, positive control and treatment with R. leguminosarum bv. viciae strain MNF-EM-R9 at molecular weight (40 kDa) .Treatments with R. leguminosarum strain MNF-EM-R4 and treatment with R. leguminosarum bv. viciae strain MNF-EM-R9 showed one band did not appear in negative, positive control, at molecular weight (85 kDa).