This study was carried out in the laboratory of tissue culture at the Research Garden, Horticulture Research Institute, Agriculture Research Center, Ministry of Agriculture; the experiments were done during the period from 2016 to 2020. This study investigated the best commercial in vitro protocol of Caladium bicolor and conservation the micro propagated shoots. The best results of surface sterilization were achieved when used 20% Clorox for 20 min. or 0.2 mg/l mercuric chloride for 5min. Also the highest value of number of shoots, shoot length and number of leaves per shoot was found at 2.0 mg/l BA or kinetin and 0.5 mg/l NAA during multiplication stage. For conservation of Caladium with sorbitol or mannitol were achieved that, number of shoots, shoot length and number of leaves per shoot was decreased by increasing the concentration of sorbitol or mannitol. The highest number of roots and root length were obtained when shoots were cultured on MS medium plus 0.2mg/l IBA during rooting stage. Peat moss + perlite media was best media for acclimatization caladium plants.