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227261

DNA-barcoding and Identification for Some Common Bean and Teparry Bean Genotypes using rbcL Gene

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Genetics and Biotechnology

Abstract

Among the different biological sources, the common bean, tepary Bean, and the wild type (Phaseolus spp < /em>.) have many biotechnological applications. The DNA barcode of the use of the rbcL gene has proven its usefulness in the study of Phaseolus spp. Phylogenetic diversity of genotypes, more than a cryptic introduction, environmental modulation, geographic distribution, and species identification in exclusive bean species. Eleven samples of common bean had been obtained from unique locations in Egypt. The gene of rbcl had proven successful in PCR reaction. Seven samples of complete array with size 4031 base pair have been obtained. The spot ranged from 574 to 575 bp with a common size of 576 bp. The identification of the species published that the samples of the specimen River Garow, Bronco, Cerdon, Goru, Giza3 three belong to Phaseolus vulgaris, and two samples of the specimen Teparry113, Teparry116 belong to Phaseolus acutifolius, respectively. The rbcL-based DNA barcode was once nearly profitable to discover distinctive specimens of Phaseolus spp < /em> according to species and genus. Some samples of the rbcL gene were no longer sufficient to perceive the stage of the genus and could not differentiate between relatively comparable species.

DOI

10.21608/assjm.2022.227261

Keywords

Barcoding, rbcL gene, phylogenetic diversity, Common and Tepary Bean, Phaseolus spp

Authors

First Name

Mohamed

Last Name

Refaat

MiddleName

Hassan

Affiliation

Faculty of Agriculture at Moshtohor, Benha University,Egypt.

Email

mohamed.refaat@fagr.bu.edu.eg

City

Toukh

Orcid

0000-0002-1555-3754

Volume

60

Article Issue

1

Related Issue

31903

Issue Date

2022-03-01

Receive Date

2022-03-27

Publish Date

2022-03-01

Page Start

119

Page End

126

Print ISSN

1110-0419

Article File

ASSJM_Volume 60_Issue 1_Pages 119-126.pdf

PDF . 621.2kB

Link

https://assjm.journals.ekb.eg/article_227261.html

Detail API

https://assjm.journals.ekb.eg/service?article_code=227261

Order

11

Type

Original Article

Type Code

841

Publication Type

Journal

Publication Title

Annals of Agricultural Science, Moshtohor

Publication Link

https://assjm.journals.ekb.eg/

MainTitle

DNA-barcoding and Identification for Some Common Bean and Teparry Bean Genotypes using rbcL Gene

Details

Type

Article

Created At

22 Jan 2023