Tracking of drinking water excellent is essential for human health. The physicochemical consequences of the water samples had been below widespread degrees except for the HCO3- cation, which was high in any respect websites. The morphological results of fifty-eight pure bacterial isolates revealed two categories (Gram-positive and negative bacteria): biochemistry, PCR sequencing, and scanning electron microscopy (SEM) showed the distinction among the 14 selected bacterial isolates that they have been chosen based on morpho-chemical consequences of the same bacterial isolates. The antibiotics sensitivity outcomes discovered that all isolates were immune to nine to twelve antibiotics from fifteen examined exceptional antibiotics. The morphophysiological alignment of sequences and SEM records confirmed a rising sort of bacterial isolates from ingesting water sources. Phylogenetic trees confirmed all fourteen isolates, Escherichia coli, Bacillus subtilis, Bacillus paralicheniformis, Bacillus licheniformis, Bacillus tequilensis, Bacillus velezensis, Pseudomonas fragi, Bacillus jeotgali, Klebsiella variicola found in special clades primarily based on genetic distance. The combination of the cutting-edge microbial monitoring tools in consuming water-based totally on DNA sequencing and SEM analysis is essential. Additionally, the current research has a look at affords a record that distinguishes among extraordinary sorts of bacteria in ingesting water.