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195296

Genetic Improvement of Catharanthus roseus L. for Abiotic Stress Tolerance using HVA-1 Gene Via Bombardment Approach

Article

Last updated: 24 Dec 2024

Subjects

-

Tags

Genetics and Biotechnology

Abstract

The main aim of this study was to introduce HVA1 gene into Catharanthus roseus callus by using PDS (Particle delivery system). In vitro seed germination of C. roseus was achieved using vigor seeds. The shootlets induction medium was MS containing 2.0 mg/l benzyl aminopurine lāˆ’1. Somatic embryogenesis was obtained by culturing various parts of explants (stem, root and leaves disc) on MS containing 2, 4-D at 3.0 mg/l and sub-cultured on MS medium containing coconut juice 3%. Genetically transformed C. roseus somatic embryos were obtained after bombardment of embryonic calli, by pAB1 plasmid harboring HVA-1 gene, salt resistance, and Bargene, herbicide resistance, coated gold particles 1.0micron, 1100 and 1350 Psi with 130 mm with HAV1 gene. The data showed that the transgenic line exhibited different levels of salt tolerance as expressed by the performance of plants dry weight, proline content and drought tolerance test. HVA-1 gene enhanced vinca salt tolerance hence enhancing the production of the important secondary metabolites from transgenic plants. The results of the present study can be seen as a step towards development of salinity tolerant transgenic vinca genotypes.

DOI

10.21608/assjm.2021.195296

Authors

First Name

Abeer

Last Name

Nagm

MiddleName

K.

Affiliation

Genetics and Genetic Engineering Dept., Faculty of Agriculture, Benha University, Egypt

Email

abeerkamal254@gmail.com

City

-

Orcid

-

First Name

Hassan

Last Name

Sherif

MiddleName

S.

Affiliation

Genetics and Genetic Engineering Dept., Faculty of Agriculture, Benha University, Egypt

Email

h.sherif@fagr.bu.edu.eg

City

-

Orcid

-

First Name

Mohamed

Last Name

Abdel-Sabour

MiddleName

Serag El-Din

Affiliation

Genetics and Genetic Engineering Dept., Faculty of Agriculture, Benha University, Egypt

Email

mohamed.abdelssabour@fagr.bu.edu.eg

City

Toukh

Orcid

-

First Name

Tamer

Last Name

El-Akkad

MiddleName

A.

Affiliation

Genetics and Genetic Engineering Dept., Faculty of Agriculture, Benha University, Egypt

Email

tamer.abdelghaffar@fagr.bu.edu.eg

City

Benha

Orcid

-

Volume

59

Article Issue

5

Related Issue

26490

Issue Date

2021-04-01

Receive Date

2021-09-20

Publish Date

2021-04-01

Page Start

489

Page End

502

Print ISSN

1110-0419

Online ISSN

2974-4830

Link

https://assjm.journals.ekb.eg/article_195296.html

Detail API

https://assjm.journals.ekb.eg/service?article_code=195296

Order

60

Type

Original Article

Type Code

841

Publication Type

Journal

Publication Title

Annals of Agricultural Science, Moshtohor

Publication Link

https://assjm.journals.ekb.eg/

MainTitle

Genetic Improvement of Catharanthus roseus L. for Abiotic Stress Tolerance using HVA-1 Gene Via Bombardment Approach

Details

Type

Article

Created At

22 Jan 2023