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194783

Short-Term Conservation of Somatic Embryo Clusters for Date Palm (Phoenix Dactylifera l.) Cv. Amry

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

HORTICULTURE

Abstract

This work aimed to develop a conservation protocol for date palm cv. Amry, an Egyptian semi dry rare cultivar. Slow growth conditions of storage studied by using low temperature at 15°C, beside the manipulation of salt strength of nutrient medium,( MS, 1/2 MS or1/4 MS) in combination with osmotic agent addition sucrose, sorbitol, or mannitol at constant concentration  (0.3 M) for each, to conserve somatic embryos clusters explants, which obtained previously through direct somatic embryogenesis of inflorescences explants. Survival percentage and recovery were evaluated after 6 months of conservation period. Results showed that conserved somatic embryos clusters explants on full MS, ½ MS, conservation medium supplemented with sucrose or sorbitol recorded the highest significant survival percentage on full MS,  ½ MS, where the lowest  significant  survival percentage was obtained when somatic embryos were  conserved on 1/4 medium supplemented with mannitol  . Best shoot number and shoot length recovery under normal growth conditions achieved on conservation medium supplemented with sucrose in comp inaction with reducing the full MS salt strength to the half for 6 months of conservation period under low temperature at 15°C.

DOI

10.21608/assjm.2021.194783

Keywords

date palm, Sorbitol, Mannitol, sucrose, Slow growth conditions, Germplasm conservation

Authors

First Name

G.A.

Last Name

Baghdady

MiddleName

-

Affiliation

Department of Horticulture , Faculty of Agriculture, Al-Azhar University, Nasr city ,Cairo, Egypt.

Email

gabdrabboh65@yahoo.com

City

-

Orcid

-

Volume

59

Article Issue

5

Related Issue

26490

Issue Date

2021-04-01

Receive Date

2021-09-15

Publish Date

2021-04-01

Page Start

295

Page End

302

Print ISSN

1110-0419

Online ISSN

2974-4830

Link

https://assjm.journals.ekb.eg/article_194783.html

Detail API

https://assjm.journals.ekb.eg/service?article_code=194783

Order

41

Type

Original Article

Type Code

841

Publication Type

Journal

Publication Title

Annals of Agricultural Science, Moshtohor

Publication Link

https://assjm.journals.ekb.eg/

MainTitle

Short-Term Conservation of Somatic Embryo Clusters for Date Palm (Phoenix Dactylifera l.) Cv. Amry

Details

Type

Article

Created At

22 Jan 2023