This investigation was carried out to study the possibility of using in vitro technique for propagation Blackberry (Rubus sp < /em>) Prime-Ark 45 cultivar. Explants of Blackberry Prime-Ark 45 cultivar were sterilized in 0.1% of sodium hypochlorite for 15 min to get aseptic culture. In starting stage, tow explants shoot tips and axillary buds were cultured on MS media supplemented with NAA at 0.0, 0.01 and 0.1 mg /L and BA at 0.0, 0.5, 1.0 and 2.0 mg /L in addition to sucrose at 3% and agar at 7 g/L. The results of starting stage showed that adding the combination between BA and NAA at 1.0 and 0.01 mg/L respectively to MS media caused the highest shoots number and shoot fresh weight compared with control and other treatments. The axillary buds were superior to shoot tips explants regarding survival percentage and other morphological characteristics. The highest shoots number and shoot fresh weight (g) were obtained when micro-shoots of tested CV., cultured on MS media supplemented with BA and KIN both at 1.0 mg /L compared with those of control and other treatments. Cytokine BA was superior to KIN regarding to mass production at multiplication stage. Halh-strenght MS media supplemented with 1.0 mg /L of NAA and IBA caused the highest root formation percentage, roots number and root length (cm) for micro-shoots of Blackberry Prime-Ark 45 variety. IBA was superior to NAA regarding roots number and root formation percentage at rooting stage. The highest root length (cm) was obtained when micro-shoots of the tested cv., were cultured on MS media supplemented with NAA at 2.0 mg /L compared with the same concentration of IBA.