Wheat (Triticum aestivum L). is one of the most crucial staple food crops in Egypt and worldwide it is adversely affected by water stress. The study aims at examine the genetic characterization of nine bread wheat genotypes, Sahel-1 (P1), Gemmiza-9 (P2), Sakha-94 (P3), Misr-1 (P4), Giza-168 (P5), L146 (P6),L133 (P7), L142 (P8) and L147 (P9) using microsatellite (SSR) markers. A total number of 48 scorable bands were amplified by five SSR primers. Thirty-four out of 48 fragments were monomorphic bands (70.83%), thus 14 loci were polymorphic (29. 17% polymorphasim).PIC value of the five SSR primers ranged from 0.863 to 0.881 with an average of 0. 870. The Nei genetic identity index ranged from 0.81 to 0.95. The genetic similarity among nine parents of wheat fell into three main clusters.